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Karen Guillemin

Karen Guillemin

Professor, Biology
Member, IMB

Ph.D., Stanford University
A.B., Harvard and Radcliffe Colleges

Office: Klamath Hall Room 249C
Office Phone: 541-346-5360
Lab: Klamath Hall Room 249
Lab Phone: 541-346-5999

Loading profile for Karen Guillemin

Research Interests

Guillemin zebrafish gut The gut microbiota of a zebrafish larva. Bacteria are in red, nuclei are in blue, and fish tissue is in green.

All animals are complex systems of interacting host and microbial cells. In the Guillemin lab, we strive to understand how hosts and their associated microbial communities shape each other. We use genetically tractable and microbiologically manipulable models systems including zebrafish and fruit flies. We explore the reciprocal impacts of microbial communities on their hosts and host environments on resident microbiota during development and in the context of disease. We perform experiments using gnotobiotic animals with defined microbial associations to uncover the causal relationships in these reciprocal interactions and to understand their mechanisms. We investigate host-microbe systems with scalable complexity, from germ-free and mono-associated animals to conventionally reared animals with their full complement of microbes. From these investigations we hope to understand the principles by which complex host-microbe systems functions and to learn how they can be manipulated to promote the health of systems like ourselves.

Microbial impacts on intestinal development

We have found that resident bacteria play important roles in the maturation of the zebrafish intestine, including promoting intestinal epithelial cell proliferation and recruiting innate immune cells in the gut. Many of these effects of the microbiota are conserved across animal species. We study the molecular mechanisms by which bacteria signal to the host to promote these changes, and the molecular pathways through which the host perceives and responds to these signals. We also investigate how host-microbe interactions can influence disease states, such as excess cell proliferation (cancer) or immune cells (inflammatory diseases).
Guillemin cell proliferation in germ-free zebrafish Cell proliferation in the epithelium of conventionally reared (A) and germ-free (B) zebrafish larvae.

Bacterial strategies for colonizing the host

We study how host-associated bacteria explore and establish residency in the environment of host tissues. We use live imaging to investigate the dynamics of bacterial colonization of the zebrafish intestine and genetic approaches to identify bacterial colonization strategies. One such strategy is chemotaxis, which we study in the gastric bacterium Helicobacter pylori that senses its extreme chemical environment with a limited repertoire of chemoreceptors. Our analysis of the structure and biochemistry of the chemoreceptor TlpB has revealed a novel pH sensing mechanism involving a urea cofactor.
Guillemin crystal structure of tlpb Structure of Helicobacter pylori chemoreceptor TlpB periplasmic domain dimer with urea molecules in pink.

Assembly of host-associated microbial communities

We investigate how microbial communities assemble in and on hosts and how these communities change during host development and disease. We survey complex, naturally assembled communities to discern the contributions of host, environmental, and stochastic processes on assembly. We also study simple, artificially constructed communities in gnotobiotic animals to understand the assembly principles that would allow us to engineer or manipulate more complex host-microbe systems.
Guillemin lab host-bacterial interaction diagram Bacterial-bacterial and bacterial-host interactions in a simple three member bacterial community in gnotobiotic zebrafish

Recent publications

(pulled from pubmed)

Recent publications

(pulled from pubmed)

Intestinal alkaline phosphatase deficiency leads to lipopolysaccharide desensitization and faster weight gain.
Yang Y, Millán JL, Mecsas J, Guillemin K
Infect Immun 2015 Jan;83(1):247-58
Immigrants in immunology: the benefits of lax borders.
Stagaman K, Martinez ES, Guillemin K
Trends Immunol 2015 May;36(5):286-9
Chemorepulsion from the Quorum Signal Autoinducer-2 Promotes Helicobacter pylori Biofilm Dispersal.
Anderson JK, Huang JY, Wreden C, Sweeney EG, Goers J, Remington SJ, Guillemin K
MBio 2015 Jul 7;6(4):e00379
Chemodetection and Destruction of Host Urea Allows Helicobacter pylori to Locate the Epithelium.
Huang JY, Sweeney EG, Sigal M, Zhang HC, Remington SJ, Cantrell MA, Kuo CJ, Guillemin K, Amieva MR
Cell Host Microbe 2015 Aug 12;18(2):147-56
Contribution of neutral processes to the assembly of gut microbial communities in the zebrafish over host development.
Burns AR, Zac Stephens W, Stagaman K, Wong S, Rawls JF, Guillemin K, Bohannan BJ
ISME J 2015 Aug 21;
The composition of the zebrafish intestinal microbial community varies across development.
Stephens ZW, Burns AR, Stagaman K, Wong S, Rawls JF, Guillemin K, Bohannan BJ
ISME J 2015 Sep 4;
A twist in the tail.
Guillemin K, Rolig AS
Elife 2014 Mar 4;3:e02386
Tending a complex microbiota requires major immune complexity.
Stagaman K, Guillemin K, Milligan-Myhre K
Mol Ecol 2014 Oct;23(19):4679-81
Spatial and temporal features of the growth of a bacterial species colonizing the zebrafish gut.
Jemielita M, Taormina MJ, Burns AR, Hampton JS, Rolig AS, Guillemin K, Parthasarathy R
MBio 2014 Dec 16;5(6)
A retrospective study of the prevalence and classification of intestinal neoplasia in zebrafish (Danio rerio).
Paquette CE, Kent ML, Buchner C, Tanguay RL, Guillemin K, Mason TJ, Peterson TS
Zebrafish 2013 Jun;10(2):228-36
Structure and proposed mechanism for the pH-sensing Helicobacter pylori chemoreceptor TlpB.
Goers Sweeney E, Henderson JN, Goers J, Wreden C, Hicks KG, Foster JK, Parthasarathy R, Remington SJ, Guillemin K
Structure 2012 Jul 3;20(7):1177-88
Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila.
Reid DW, Muyskens JB, Neal JT, Gaddini GW, Cho LY, Wandler AM, Botham CM, Guillemin K
Front Cell Infect Microbiol 2012;2:24
Investigating bacterial-animal symbioses with light sheet microscopy.
Taormina MJ, Jemielita M, Stephens WZ, Burns AR, Troll JV, Parthasarathy R, Guillemin K
Biol Bull 2012 Aug;223(1):7-20
Epithelial cell proliferation in the developing zebrafish intestine is regulated by the Wnt pathway and microbial signaling via Myd88.
Cheesman SE, Neal JT, Mittge E, Seredick BM, Guillemin K
Proc Natl Acad Sci U S A 2011 Mar 15;108 Suppl 1:4570-7
Helicobacter pylori perceives the quorum-sensing molecule AI-2 as a chemorepellent via the chemoreceptor TlpB.
Rader BA, Wreden C, Hicks KG, Sweeney EG, Ottemann KM, Guillemin K
Microbiology 2011 Sep;157(Pt 9):2445-55
A gastric pathogen moves chemotaxis in a new direction.
Sweeney EG, Guillemin K
MBio 2011;2(5)
Study of host-microbe interactions in zebrafish.
Milligan-Myhre K, Charette JR, Phennicie RT, Stephens WZ, Rawls JF, Guillemin K, Kim CH
Methods Cell Biol 2011;105:87-116
A greasy foothold for Helicobacter pylori.
Wandler AM, Parthasarathy R, Guillemin K
Cell Host Microbe 2010 May 20;7(5):338-9
Natural transformation increases the rate of adaptation in the human pathogen Helicobacter pylori.
Baltrus DA, Guillemin K, Phillips PC
Evolution 2008 Jan;62(1):39-49
Bugs inside Bugs: what the fruit fly can teach us about immune and microbial balance in the gut.
Muyskens JB, Guillemin K
Cell Host Microbe 2008 Mar 13;3(3):117-8
We know you are in there: conversing with the indigenous gut microbiota.
Cheesman SE, Guillemin K
Res Microbiol 2007 Jan-Feb;158(1):2-9
The quorum-sensing molecule autoinducer 2 regulates motility and flagellar morphogenesis in Helicobacter pylori.
Rader BA, Campagna SR, Semmelhack MF, Bassler BL, Guillemin K
J Bacteriol 2007 Sep;189(17):6109-17
Early developmental pathology due to cytochrome c oxidase deficiency is revealed by a new zebrafish model.
Baden KN, Murray J, Capaldi RA, Guillemin K
J Biol Chem 2007 Nov 30;282(48):34839-49